中国食品学报2016，Vol.16Issue(5)：147-154,8.DOI:10.16429/j.1009-7848.2016.05.021
转基因抗除草剂玉米DAS-40278-9品系特异实时荧光定量PCR检测方法的建立
Establishment of Event-specific Quantitative PCR of Event DAS-40278-9 Herbicide-tolerant Maize
摘要
Abstract
Event-specific quantitative PCR detection method of genetically modified maize (Event DAS-40278-9) was established.PCR primers and Taqman probe were designed according to the flanking sequence of exogenous gene of Event DAS-40278-9 by the ABI Primer Express 3.0.Sample containing 1％ of Event DAS-40278-9 was tested.The resuits showed that the standard curve obtained was credible through the slope,the correlation coefficient,amplification efficiency.The detection result of sample was 1.04％,closed to the true content.It was proved that the specificity,sensitivity and precision of this method was relatively high and could be used to test GMO samples.关键词
DAS-40278-9/zSSIIb/旁侧序列/定量PCR/检测Key words
event DAS-40278-9/zSSIIb/flanking sequence/quantitative PCR/detection引用本文复制引用
尹全,李忆,王东,张富丽,刘文娟,常丽娟,宋君..转基因抗除草剂玉米DAS-40278-9品系特异实时荧光定量PCR检测方法的建立[J].中国食品学报,2016,16(5):147-154,8.基金项目
四川省质监局标准化技术体系研究项目(ZY-BZ2013-39) （ZY-BZ2013-39）
四川省财政现代农业技术创新与示范专项(2014CXSF-040) （2014CXSF-040）
四川省教育厅自然科学一般项目(15ZB0331) （15ZB0331）
