食品科学技术学报2016，Vol.34Issue(4)：31-38,8.DOI:10.3969/j.issn.2095-6002.2016.04.006
解淀粉芽孢杆菌GSB a-1产凝乳酶培养基组成的优化
Optimization by Response Surface Methodology of Medium Composition for Producing Milk-clotting Enzyme by Bacillus Amyloliquefaciens GSBa-1
摘要
Abstract
A Bacillus amyloliquefaciens strain GSBa-1 was isolated from sweet Jiuqu. In order to improve the ability of producing a milk-clotting enzyme (MCE) by liquid fermentation of strain GSBa-1, the sin-gle factor test and response surface methodology were employed to optimize the composition of the medium used. The single factor experiment was carried out to analyze the influence of different carbon source, ni-trogen source, metal salt and phosphorus source on production of MCE by strain GSBa-1. Then the Box-Behnken design based RSM experiment was used to study the optimum combination of three main factors such as the content of maltose, peptone and yeast extract powder in the medium. The optimal medium composition for producing MCE by GSBa-1 was as follows:maltose 1. 93 g/L, peptone 10. 89 g/L, and yeast extract powder 2. 15 g/L. When the optimal culture medium was used, MCE activity was up to (562. 57 ± 7. 67)Su/mL, which is similar as the theoretical prediction value of 537. 10 Su/mL with an aver-age error of 4. 53%. After optimization of the medium composition, the MCE activity produced by strain GSBa-1 increased by 1. 88 times compared with that of the basal medium.关键词
解淀粉芽孢杆菌GSBa-1/凝乳酶/响应面法/培养基组成Key words
Bacillus amyloliquefaciens strain GSBa-1/milk-clotting enzyme/response surface method-ology/medium composition分类
轻工业引用本文复制引用
腾军伟,杨贞耐..解淀粉芽孢杆菌GSB a-1产凝乳酶培养基组成的优化[J].食品科学技术学报,2016,34(4):31-38,8.基金项目
国家自然科学基金面上项目(31371804) （31371804）
北京市百千万人才工程资助项目(B类)。 （B类）
