食品科学2016，Vol.37Issue(13)：19-24,6.DOI:10.7506/spkx1002-6630-201613004
金蝉花多糖的抗氧化活性及结构分析
Antioxidant Activity and Preliminary Structure Analysis of Polysaccharides from Cordyceps cicadas
摘要
Abstract
In this study, the antioxidant activities and structural characteristics of polysaccharides from Cordyceps cicadae were determined. Two crude polysaccharide fractions (CP50 and CP80) were extracted from Cordyceps cicadae with hot water and isolated by precipitation with different concentrations of ethanol. Their antioxidant activities were also analyzed. The results indicated that both CP50 and CP80 were effective in antioxidant activity. The radical scavenging activity of CP50 was higher than that of CP80. CP50 also showed higher reducing capacity and total antioxidant activity. CP50 was then further purified by gel filtration on diethylaminoethyl (DEAE)-cellulose-52 and Sephadex G-100 columns, and two purified fractions were obtained, namely CPA-1 and CPB-1. Ultraviolet spectroscopy and high-performance liquid chromatography (HPLC) analysis revealed that CPA-1 and CPB-1 were homogeneous. Monosaccharide analysis revealed that both CPA-1 and CPB-1 were composed of Glc, Gal and Man with a molar ratio of 1:0.48:0.52 and 1:0.14:0.114, respectively. Moreover, both polysaccharide fractions had three helix structures.关键词
金蝉花多糖/抗氧化活性/分离纯化/结构分析Key words
Cordyceps cicadaes polysaccharides/antioxidant activity/separation and purification/structural analysis分类
中医学引用本文复制引用
封燕,贡小辉,韦德群,刘英坤,赵明,于小凤,OPEYEMI Joshua Olatunji,焦心怡,欧阳臻..金蝉花多糖的抗氧化活性及结构分析[J].食品科学,2016,37(13):19-24,6.基金项目
国家自然科学基金面上项目（81072985；81373480；81573529）；江苏省大学生实践创新训练计划立项项目（201513986001Y）；江苏大学第13批大学生科研立项一般项目（13A154）；江苏大学第14批大学生科研立项一般项目 （）
