食品科学2016，Vol.37Issue(13)：36-41,6.DOI:10.7506/spkx1002-6630-201613007
菜籽多肽体外和细胞内抗氧化性评价及氨基酸分析
Antioxidant Activities in Vitro and in Cells and Amino Acid Composition of Rapeseed Peptides
摘要
Abstract
The rapeseed protein hydrolysate (RPH) obtained by enzymatic hydrolysis of double-zero rapeseed with Alcalase 2.4L was separated by ultrafiltration and gel filtration chromatography to produce active peptide fractions. The antioxidant activity of RPH and its active peptides was evaluated in vitro. Furthermore, the amino acid composition of the most potent antioxidant fraction was analyzed. The results showed that RPH was separated into 4 fractions in different molecular weight ranges. RPH-P4 (molecular weight<3 kD) had the strongest antioxidant capacity among these peptide fractions. RPH-P4 was further separated by gel filtration on Sephadex G-15 into 3 peaks. The antioxidant capacity of RPH-P4-S3 was far higher than that of 2 other peaks (P<0.05). Its oxygen radical absorbance capacity (ORAC) value was (1 951.90 ± 20.35) µmol TE/g sample, cellular antioxidant activity (CAA) value (143.38 ± 4.11) µmol QE/g sample, and EC50 value (45.63 ± 3.67) µg/mL. The content of antioxidant amino acids in RPH-P4-S3 was as high as 72.90%, and the essential amino acid content was 53.52%. This research suggests that RPH-P4-S3, produced by enzymatic hydrolysis of double-zero rapeseed with Alcalase 2.4L, has high antioxidant activity and nutritional value and thus can be applied in the development of antioxidant-related functional foods and healthcare products as a functional ingredient.关键词
菜籽多肽/抗氧化能力指数/细胞抗氧化能力/氨基酸分析Key words
rapeseed peptides/oxygen radical absorbance capacity/cellular antioxidant activity/amino acid analysis分类
轻工业引用本文复制引用
张晶,张怡一,徐斐然,石嘉怿,鞠兴荣,王立峰..菜籽多肽体外和细胞内抗氧化性评价及氨基酸分析[J].食品科学,2016,37(13):36-41,6.基金项目
国家重点研发计划项目（2016YFD0401401）；国家高技术研究发展计划（863计划）项目（2013AA102207）；国家农业成果转化计划项目（2014GB2C100318）；江苏省产学研前瞻性联合研究项目（BY2016009-01）；江苏省高校自然科学基金项目（14KJB550004）；江苏高校优势学科建设工程资助项目；南京财经大学研究生科技创新计划资助项目 （）
