食品科学2016，Vol.37Issue(13)：60-64,5.DOI:10.7506/spkx1002-6630-201613011
大米镉结合蛋白的分离纯化及纯度鉴定
Separation, Purification, and Purity Identification of Cd-Binding Protein from Rice
摘要
Abstract
A graphite furnace atomic absorption spectrometry (GFAAS) method was used to determine the cadmium content in rice grains from different varieties and at milling levels, as well as in four rice seed storage proteins (albumin, globulin, prolamin and glutelin) obtained through the Osborne sequential extraction method. Moreover, rice cadmium-binding protein (RCBP) was separated and purified to homogeneity by using ultrafiltration and ion exchange chromatography. The purity and molecular mass of RCBP were identified. The results showed that the cadmium content in indica rice was higher than that in glutinous rice and japonica rice and the cadmium content in rice decreased with the increase of milling level. The cadmium contents of rice albumin, globulin, prolamin, and glutelin were 0.66, 0.31, 0.63 and 0.23μg/g, respectively. RCBP (fraction c) with high purity and good homogeneity as well as molecular mass of 14 kD was prepared from rice albumin (RA).关键词
大米镉结合蛋白/分离纯化/离子交换色谱/分子质量Key words
rice Cd-binding protein/separation and purification/ion exchange chromatography/molecular mass分类
轻工纺织引用本文复制引用
陈露,陈季旺,蔡俊,丁文平,吴永宁..大米镉结合蛋白的分离纯化及纯度鉴定[J].食品科学,2016,37(13):60-64,5.基金项目
粮食公益性行业科研专项（201513006-3）；武汉轻工大学重大项目培育专项（2011Z05）；武汉市国际合作项目（201231234466）；湖北省自然科学基金面上项目（2014CFB888）；武汉轻工大学研究生创新基金项目 （）
