食品科学2016，Vol.37Issue(13)：89-94,6.DOI:10.7506/spkx1002-6630-201613016
冬枣多糖的分离纯化及抗氧化活性研究
Isolation, Purification and Antioxidant Activity of Polysaccharides from Zizyphus jujube cv. Dongzao
摘要
Abstract
Objective: To study the monosaccharide composition, structure and antioxidant activity of polysaccharide fractions isolated and purified from Zizyphus jujube cv. Dongzao. Methods:The extraction of polysaccharides was performed by water extraction and subsequent ethanol precipitation, and after removal of proteins and pigments, the polysaccharides were purified by successive DEAE-52 cellulose column and Sephadex G-100 gel column chromatography. The purity and molecular weights of the purified polysaccharides were determined by Sephadex G-100 column chromatography. Preliminary structural characterization was carried out by ultraviolet-visible (UV-VIS) spectroscopy, infrared (IR) spectroscopy and gas chromatography (GC). The antioxidant activity of the crude and purified polysaccharides was evaluated using the phenanthroline method and 1, 1-diphenyl-2-picrylhydroxyl (DPPH) radical scavenging activity assay. Results:Two polysaccharide fractions (DPA and DPB) were purified to homogeneity from the crude polysaccharides as identified by Sephadex G-100. The molecular weight of DPA and DPB was 1.04 × 104 and 3.02 × 105 D, respectively. DPA and DPB did not contain protein or nucleic acid, but contained the pyranose ring skeleton glucoside. The monosaccharide composition of DPA consisted of arabinose, mannose, glucose and galactose with a molar ratio of 6.66:1.00:6.75:2.09, whereas that of DPB consisted of rhamnose, arabinose, mannose, glucose and galactose with a molar ratio of 4.33:10.90:1.00:3.25:4.78. The crude polysaccharides, DPA and DPB all had antioxidant activity in a concentration dependent manner. The scavenging percentages of DPA and DPB at 8 mg/mL for hydroxyl radical were 28.52%and 78.79%respectively, which were 9.97%and 24.54%at 0.4 mg/mL, respectively. Conclusion:Both DPA and DPB had antioxidant activity.关键词
冬枣/多糖/分离纯化/结构鉴定/抗氧化活性Key words
Zizyphus jujube cv. Dongzao/polysaccharide/isolation and purification/structure identification/antioxidant activity分类
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潘莹,许经伟..冬枣多糖的分离纯化及抗氧化活性研究[J].食品科学,2016,37(13):89-94,6.基金项目
山东省高校科研计划项目 （）
