食品科学2016，Vol.37Issue(13)：136-139,4.DOI:10.7506/spkx1002-6630-201613024
基于PCR-DGGE方法分析榨菜中乳酸菌群落结构
PCR-DGGE Analysis of Lactic Acid Bacterial Community Structure in Pickled Mustard Tuber
摘要
Abstract
In order to understand the diversity of lactic acid bacteria in pickled mustard tuber and its influencing factors, two commercial bagged pickled mustard tubers (with and without hot pepper) were determined for the contents of salt and nitrite, and the V7-V8 region of total bacterial 16S rDNA sequences was separated by denaturing gradient gel electrophoresis (DGGE). Furthermore, the species richness, evenness and diversity of lactic acid bacteria were analyzed by the Quantity One software. Results indicated that the contents of salt and nitrite in the first sample were slightly lower than in the second sample. However, no significant difference in species richness, evenness and diversity was observed between both samples (P>0.05). The target DGGE bands were recovered, cloned and sequenced by comparison with the sequences published in the GenBank after PCR amplification. A total of 5 bands from the DGGE gel of the second sample were recovered and identified as Pediococcus argentinicus CRL 776, uncultured Lactobacillus sp. isolate DGGE gel band lx12, uncultured bacterium clone 11.02-12, uncultured bacterium clone 11.02-9, and uncultured Lactobacillus sp. clone PxSC03 with a similarity of 98%, 96%, 97%, 97%, and 97%, respectively. Four bands from the first sample were recovered and identified as uncultured Lactobacillus sp., Lactobacillus sakei A156, Lactobacillus sakei YY1, and Lactobacillus sakei WJ1 with a similarity of 96%, 97%, 98%and 97%, respectively. These results show that hot pepper has little effect on the microbial community structure of pickled mustard tuber.关键词
榨菜/亚硝酸盐/乳酸菌/聚合酶链式反应-变性梯度凝胶电泳Key words
pickled mustard tuber/nitrite/lactic acid bacteria/polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE)分类
轻工纺织引用本文复制引用
燕平梅,乔宏萍,赵文婧,单树花,王琪,柴政,陈燕飞..基于PCR-DGGE方法分析榨菜中乳酸菌群落结构[J].食品科学,2016,37(13):136-139,4.基金项目
国家自然科学基金面上项目（31171743）；山西省基础条件平台项目 （）
