食品科学2016，Vol.37Issue(13)：153-156,4.DOI:10.7506/spkx1002-6630-201613027
假肠膜明串珠菌甘露醇脱氢酶基因的克隆及表达
Cloning and Expression of Mannitol Dehydrogenase Gene from Leuconostoc pseudomesenteroides
摘要
Abstract
The structural gene encoding mannitol dehydrogenase (MDH) from Leuconostoc pseudomesenteroides was amplified by PCR and cloned into the vector pETDuet-1. As a result, the plasmid pETDuet-1-mdh was constructed and transformed into E. coli BL21(DE3) for MDH expression induced by isopropyl-β-D-thiogalactoside (IPTG). The length of the mdh structural gene was l 017 bp. The recombinant mdh gene was successfully expressed in Escherichia coli and the molecular weight of the expressed protein was 36.0 kD. The activity of recombinant mannitol dehydrogenase was 0.15 U/mg pro, which was higher than the MDH activity of Leuconostoc pseudomesenteroides (0.03 U/mg pro).关键词
甘露醇/甘露醇脱氢酶/假肠膜明串珠菌/基因克隆/表达Key words
mannitol/mannitol dehydrogenase/Leuconostoc pseudomesenteroides/gene cloning/expression分类
轻工纺织引用本文复制引用
程雅韵,王新,郑琳,李官浩,崔虎山,金清..假肠膜明串珠菌甘露醇脱氢酶基因的克隆及表达[J].食品科学,2016,37(13):153-156,4.基金项目
国家自然科学基金地区科学基金项目（31260362）；吉林省教育厅“十二五”科学技术研究项目 （）
