食品科学2016，Vol.37Issue(14)：1-5,5.DOI:10.7506/spkx1002-6630-201614001
虾壳中复合钙粉的制备工艺优化及大鼠对其吸收效果的评价
Preparation of Blended Calcium Powders from Shrimp Shell and Evaluation of Its Calcium Absorption in Rats
摘要
Abstract
Purpose:To optimize the preparation of blended calcium powders from shrimp shell and to evaluate its calcium absorption in rats. Methods: The extraction of blended calcium powders from shrimp shell (Penaeus vannamei) by the combined use of trypsin and citric acid was investigated. Furthermore, the effects of calcium powder on growth, calcium absorption in rats was studied by establishing calcium deficiency model. Results: The optimized preparation conditions were determined as follows: initial hydrolysis with 2.5 × 103 U/g of trypsin at pH 8.0 and 45 ℃ for 1.5 h followed by treatment with 20 mL of 0.35 mol/L citric acid for 1.5 h. Under these conditions, the rate of proteolysis was 57.24%, and the dissolution rate of calcium was 85.78%. Furthermore, the calcium power contained 19.6%calcium and 18.3%total amino acids, which was a hazel powder with a delicate texture and without off-flavor. Animal experiments showed that the calcium powder prepared from shrimp shell significantly improved calcium absorption in rats (P<0.05), and that gavage dose of 66.5 mg/(kg·d) (calculate based on calcium content and the body weight of rats) could improve the contents of calcium and phosphorous in serum. Conclusion:Trypsin combined with citric acid offers a mild and effective way to extract blended calcium powder from shrimp shell, which exhibits better absorption than of CaCO3 with the same calcium content.关键词
南美白对虾虾壳/复合钙粉/胰蛋白酶/柠檬酸Key words
shrimp shell/blended calcium powders/trypsin/citric acid分类
轻工业引用本文复制引用
张文静,陈舜胜..虾壳中复合钙粉的制备工艺优化及大鼠对其吸收效果的评价[J].食品科学,2016,37(14):1-5,5.基金项目
国家自然科学基金面上项目（31471685）；“十二五”国家科技支撑计划项目（2012BAD28B05）；融合海洋食品科学的海洋生物药物项目（B5201120040）；上海市2015高校内涵建设项目 （）
