食品科学2016，Vol.37Issue(14)：18-23,6.DOI:10.7506/spkx1002-6630-201614004
响应面试验优化果胶酶辅助提取锁阳原花青素工艺
Optimization of Pectinase-Assisted Extraction of Proanthocyanidin from Cynomorium songaricum Rupr. Using Response Surface Methodology
摘要
Abstract
Purpose: To optimize the pectinase-assisted extraction of proanthocyanidins (PC) from the stems of Cynomorium songaricum Rupr.. Methods:Pectinase was introduced into the process for improving the extraction efficiency. The extraction conditions were optimized by using response surface methodology (RSM). Five key factors including enzyme dosage, hydrolysis duration, pH value, temperature and ethanol concentration were selected by one-factor-at-a-time experiments. The extraction yield of PC was used as the response. Results:A quadric regression equation for predicting the extraction yield of PC was established using Box-Behnken design and the extraction conditions were optimized as follows:hydrolysis for 34 min with 1%pectinase at 52℃and initial pH 4.8 followed by extraction for 1.5 h with 70%ethanol as the extraction solvent. Under these optimized conditions, the extraction yield of PC reached 14.30%. Conclusion:The optimized pectinase-assisted extraction method can enhance the extraction efficiency of PC from Cynomorium songaricum Rupr..关键词
锁阳/果胶酶/原花青素/响应面法Key words
Cynomorium songaricum Rupr./pectinase/proanthocyanidins/response surface methodology分类
化学工程引用本文复制引用
崔玮,张勇,高海宁,焦杨,李彩霞..响应面试验优化果胶酶辅助提取锁阳原花青素工艺[J].食品科学,2016,37(14):18-23,6.基金项目
国家中医药管理局2012年中医药行业科研专项 （）
