食品科学2016，Vol.37Issue(14)：51-55,5.DOI:10.7506/spkx1002-6630-201614009
灵芝孢子粉破壁工艺优化及其抗肿瘤作用
Optimization of Disruption of Ganoderma lucidum Spores and Antitumor Effect of the Broken Spores
摘要
Abstract
The purpose of this study was to investigate the optimum conditions for breaking Ganoderma lucidum spores by high-pressure homogenization using water as the solvent and to evaluate the antitumor effect of the broken spores. The optimization of three process variables including solid-to-liquid ratio, homogenization pressure, and the number of homogenization cycles for improved percentage of broken spores was conducted through one-factor-at-a-time and orthogonal array experiments. S180 tumor-bearing mice models were established for evaluation of the antitumor effect of the broken G. lucidum spores. The results showed that the optimal conditions for breaking G. lucidum spores were obtained as follows:homogenization pressure, 150 MPa;homogenization cycles, 3 times;and solid-to-liquid ratio, 1:100 (g/mL). Under these conditions, the percentage of broken spores could be up to 94.35%. Antitumor tests showed that the percentage inhibition of tumor growth in S180 tumor-bearing mice by the broken spores was 43.37%–57.59%, and the liver index and lung index were little affected while the thymus index and spleen index were significantly enhanced. Therefore, broken G. lucidum spores possessed good antitumor effect.关键词
灵芝孢子/破壁/高压均质法/抗肿瘤作用Key words
Ganoderma lucidum spores/spore disruption/high pressure homogenization method/antitumor effect分类
中医学引用本文复制引用
刘春延,张国财,程方志,张国珍,赵博,林连男..灵芝孢子粉破壁工艺优化及其抗肿瘤作用[J].食品科学,2016,37(14):51-55,5.基金项目
哈尔滨市科技局科技项目（2014FF6CJ002）；中央高校基本科研业务费专项 （）
