食品科学2016，Vol.37Issue(14)：74-79,6.DOI:10.7506/spkx1002-6630-201614013
牛初乳中免疫球蛋白G双抗夹心ELISA检测方法的建立
Development of Double Antibody Sandwich ELISA for Detection of Bovine Colostrum Immunoglobulin G
摘要
Abstract
The hybridoma cells secreting monoclonal antibody (McAb) against bovine colostrum immunoglobulin G (IgG) were developed by cell fusion technology after immunization of BALB/c mice with bovine IgG. McAbs were obtained by purification of mouse ascites. A double antibody sandwich enzyme-linked immunosorbent assay (ELISA) technique was established for the detection of bovine colostrum IgG. The linear range was 7.8–1 000 ng/mL and the limit of detection (LOD) was 7.06 ng/mL. The intra-assay and inter-assay coefficient of variations were 4.52%and 4.94%, respectively. The recovery rate was 91.85%–102.45%. The ELISA method can provide a simple, accurate and stabile approach for the rapid detection of bovine colostrum IgG.关键词
牛初乳/免疫球蛋白G/单克隆抗体/双抗夹心酶联免疫吸附法Key words
bovine colostrum/immunoglobulin G (IgG)/monoclonal antibody/sandwich ELISA分类
轻工纺织引用本文复制引用
刘金,王丽威,岳喜庆..牛初乳中免疫球蛋白G双抗夹心ELISA检测方法的建立[J].食品科学,2016,37(14):74-79,6.基金项目
2011年度国家星火计划项目 （）
