食品科学2016，Vol.37Issue(14)：149-153,5.DOI:10.7506/spkx1002-6630-201614026
高效液相色谱法同时测定枣果实中的有机酸和VC含量
Simultaneous Determination of Organic Acids and Vitamin C in Jujube Fruits by HPLC
摘要
Abstract
Objective:This study aimed to establish and validate a high performance liquid chromatographic (HPLC) method for the fast simultaneous determination of organic acids and vitamin C (VC) in jujube fruits. Methods: The analytes in samples were extracted ultrasonically in a water bath, centrifugated and purified by membrane filtration for analysis by HPLC with diode-array detection (DAD). The chromatographic column used was Inertsil ODS-3 (4.6 mm × 250 mm, 5μm). The separation was performed using 0.04 mol/L KH2PO4 (pH 2.40) as mobile phase at 0.5 mL/min flow rate by isocratic elution. The column temperature and detection wavelength were set as 30 ℃ and 210 nm, respectively. Results: Under these chromatographic conditions, 7 organic acids and VC could be successfully separated in 30 minutes. The coefficients of determination (R2) of the standard calibration curves of the analyzed compounds were all above 0.998 9 and the precision expressed as relative standard deviations were between 0.98%and 4.77%(n=5) and the repeatability RSDs were in the range of 0.88%to 2.19%(n=5). The mean recovery rates of seven organic acids and vitamin C were in the range of 91.35%to 105.74%. Conclusion:This method is an accurate, rapid, and applicable method to determine organic acids and vitamin C in jujube fruits.关键词
高效液相色谱法/枣/有机酸/VCKey words
high performance liquid chromatography (HPLC)/jujube/organic acid/vitamin C分类
农业科技引用本文复制引用
马倩倩,吴翠云,蒲小秋,王德,孙亚强..高效液相色谱法同时测定枣果实中的有机酸和VC含量[J].食品科学,2016,37(14):149-153,5.基金项目
兵团科技创新团队建设项目（2014CC006）；兵团产学研重大专项（2013AA001-1）；塔里木大学校长基金育种专项 （）
