食品科学2016，Vol.37Issue(14)：203-206,4.DOI:10.7506/spkx1002-6630-201614037
乙酸酐衍生GC-ECD法测定饮料中三氯蔗糖含量
Determination of Sucralose in Drinks by Gas Chromatography with Electron Capture Detector (GC-ECD) after Derivatization with Acetic Anhydride
黄志勇 1陈艳 1冯慧 1刘超 1景赞1
作者信息
- 1. 乐山市食品药品检验检测中心，四川 乐山 614000
- 折叠
摘要
Abstract
A gas chromatographic (GC) method with an electron capture detector (ECD) after acetylation was developed for the determination of sucralose in drinks. Samples were diluted with water and then derivatized with acetic anhydride in the presence of 1-methylimidazole as a catalyst. Qualitative and quantitative analyses of the derivatives were achieved by GC-MS and GC-ECD, respectively. The results showed that the target derivative was 4,1’,6’-trichloro-2,3,6,3’,4’-sucrose pentaacetate (TOSPA). The calibration curve for the analyte showed good linearity in the range of 0.02–1.2 mg/mL (R=0.999 4). The limit of detection (LOD) for sucralose was 0.23 mg/kg, and the recoveries obtained by standard addition method were between 90.0% and 94.5%, with relative standard deviations (RSD, n = 6) between 0.6% and 2.5%. This method provides the advantages of low LOD, high sensitivity, less disturbance, good linearity, high accuracy and simple operation. Thus, it can be applied in sucralose analysis in drinks.关键词
三氯蔗糖/乙酸酐衍生/气相色谱-电子捕获器/气相色谱-质谱联用Key words
sucralose/derivatization with acetic anhydride/gas chromatography-electron capture detector (GC-ECD)/gas chromatography-mass spectrometry (GC-MS)分类
化学化工引用本文复制引用
黄志勇,陈艳,冯慧,刘超,景赞..乙酸酐衍生GC-ECD法测定饮料中三氯蔗糖含量[J].食品科学,2016,37(14):203-206,4.
