茶叶科学2016，Vol.36Issue(4)：363-371,9.
茶黄素对 BSA/MDA 羰-氨交联反应体系中的蛋白质羰基化及其聚集化抑制作用研究
Inhibition of Theaflavins on Protein Carbonylation and Aggregation in the System of BSA/MDA Carbonyl-ammonia Cross Linking Reaction in vitro
摘要
Abstract
A model of age pigment generation was builted by carbonyl-ammonia cross linking reaction in vitro, the datas indicate that theaflavins can not only inhibit the generation of age pigment in protein carbonylation, but also supress β-sheet-rich in aggregate protein in this model by thioflavin T-based fluorometric assay, scanning electron microscope, transmission electric microscope, SDS-PAGE and NBT staining, there are postive relationship between protein carbonylation on mechanism of aging and protein aggregation that lead to neurodegenerative disease.关键词
茶黄素/羰-氨交联/老年色素/蛋白质聚集化Key words
theaflavin/carbonyl-ammonia cross linking reaction/age pigment/protein aggregation分类
农业科技引用本文复制引用
张静,蔡淑娴,黄建安,李娟,钟妮,阳衡,刘仲华..茶黄素对 BSA/MDA 羰-氨交联反应体系中的蛋白质羰基化及其聚集化抑制作用研究[J].茶叶科学,2016,36(4):363-371,9.基金项目
国家自然科学基金项目（31471590）、湖南省研究生科研创新项目（CX2015B245）、国家茶叶产业技术体系项目（CARS-23-11B）。 （）
