茶叶科学2016,Vol.36Issue(4):432-439,8.
茶树炭疽病抗性的 QTL 分析
QTL Analysis for Anthracnose Resistance in Tea Plant (Camellia sinensis)
摘要
Abstract
In order to provide a basis for breeding tea plants with anthracnose resistance, 170 F1 plants, derived from LJ43♀×BHZ♂, were used to constructed a linkage map by SSR markers. Field observation and indoor test were carried out in 2014. The data of phenotypic characters were used for QTL mapping and analysis by the method of MQM mapping. A pathogen was isolated from a diseased leaf of the F1 plants, and its gene sequence of ITS had 99%similarity with Colletotrichum sp. based on NCBI BLAST. The plants grown in open field were more easy to be infected by the pathogen than those grown in rooms. Eight QTLs were detected in six different linkage groups by QTL analysis. The LOD and PVE of individual QTLs ranged from 2.53 to 6.80 and 5.6% to 13.8%, respectively. A main QTL with LOD 6.80 and PVE 13.8% was detected in LG10.关键词
炭疽病抗性/QTL 定位/茶树/SSR 遗传图谱Key words
anthracnose resistance/QTL mapping/tea plant/SSR linkage map分类
农业科技引用本文复制引用
徐礼羿,谭礼强,王丽鸳,齐桂年,成浩,韦康..茶树炭疽病抗性的 QTL 分析[J].茶叶科学,2016,36(4):432-439,8.基金项目
四川省茶树育种科技专项(2012-12CGZHZX0579)、浙江省茶树农业新品种选育重大科技专项(2012C12905)、国家茶叶产业技术体系(nycytx-23)。 ()