粮油食品科技2016，Vol.24Issue(5)：38-43,6.
高效液相色谱法测定米糠油中谷维素
Determination of oryzanol in rice bran oil by high performance liquid chromatography
摘要
Abstract
An analytical method based on solid phase extraction - high performance liquid chromatography was established for the determination of six oryzanol compositions and total oryzanol content in rice bran oil. The samples were purified by diol - silica gel series SPE column. The standard sample (γ - Oryza-nol)was used for quantification. The chromatographic column was Agilent ZORBAX Eclipse Plus C18 (250 mm × 4. 6 mm,5 μm)and the mobile phase was acetonitrile with 1% acetic acid∶ isopropanol =95∶ 5. The wavelength of UV was 321 nm. The average recoveries of six compositions and total content were in the range of 95. 6% ~ 105. 7% and 97. 8% ~ 100. 2% with relative standard deviations of 0. 1% ~4. 6% and 0. 7% ~ 3. 4% . The method was suitable for determination of oryzanol composition and content in rice bran oil,which provided technical support for further research of oryzanol.关键词
谷维素/高效液相色谱/米糠油/阿魏酸酯Key words
oryzanol/high performance liquid chromatography/rice bran oil/ferulic acid ester分类
轻工纺织引用本文复制引用
朱琳,薛雅琳,张东,柴杰,安骏,刘俊含..高效液相色谱法测定米糠油中谷维素[J].粮油食品科技,2016,24(5):38-43,6.基金项目
碾米制粉制油节粮节能智能新装备研发与示范 （）
