食品科学2016，Vol.37Issue(18)：13-18,6.DOI:10.7506/spkx1002-6630-201618003
复合重组磷脂酶用于大豆油脱胶的工艺优化
Optimization of Soybean Oil Degumming by Mixed Recombinant Phospholipases
摘要
Abstract
Crude soybean oil was degummed by mixtures of recombinant phospholipasesA1 (PLA1) andphospholipase C(PLC), which were expressed successfully in the recombinantE. coli.by our laboratory.We investigated the effects of different parameters, including temperature, pH, reaction time, water dosage, stirring rate and enzyme dosage on degumming efficiency. Commercial PLA1(Lecitase Ultra™) was used for comparison. Then, these parameters were optimized by orthogonal array design. The optimal conditions were confirmed as follows: reaction temperature, 45℃; pH, 6.5; reaction time, 3 h; stirring speed, 300 r/min; PLA1 dosage, 7 940 U/kg; and PLC dosage, 23 130 U/kg. The degumming efficiency obtained with the mixed recombinant enzymes was comparable to that obtained with Lecitase Ultra™. The final phosphorus content of degummed soybean oil was lower than 5 mg/kg, which met the requirements of physical refining. This study laid the foundation for developing food-grade enzyme preparations with self-owned intellectual property rights of cooking oil degumming.关键词
重组大肠杆菌/复合磷脂酶/大豆油/酶法脱胶/正交试验Key words
recombinantE. coli/mixed phospholipases/soybean oil/enzymatic degumming/orthogonal array experiment分类
生物科学引用本文复制引用
程实,王长坤,张梁,李赢,石贵阳,杨盛荣,陈国安..复合重组磷脂酶用于大豆油脱胶的工艺优化[J].食品科学,2016,37(18):13-18,6.基金项目
国家高技术研究发展计划（863计划）项目（2011AA100905）；教育部“新世纪优秀人才支持计划”项目（NCET-11-0665）；江南大学食品科学与技术国家重点实验室自由探索课题项目 （）
