食品科学2016，Vol.37Issue(18)：19-25,7.DOI:10.7506/spkx1002-6630-201618004
复合酶法提取石榴籽多糖的工艺优化
Optimization of Enzymatic Extraction of Polysaccharides from Pomegranate Seeds with Mixed Enzymes
摘要
Abstract
In this study, we optimized the conditions for enzymatic extraction of polysaccharides from pomegranate seeds with a mixed enzyme preparation consisting of pectinase, cellulase and mannase. The optimization of the mixing ratio of the three enzymes was performed by the combined use of one-factor-at-a-time method andD-optimal mixture design. Thereafter, the enzymatic extraction conditions were optimized by response surface methodology using Box-Behnken design. The yield of polysaccharides was taken as response for both optimizations. It was shown that the optimal combination of enzymes were 24.2% pectinase, 67.2% cellulase and 8.6% mannose and that the optimal extraction conditions were determined as follows: liquid/solid ratio, 20:1 (mL/g); initial pH, 4.5; hydrolysis temperature, 48.5℃; and hydrolysis time, 287 min. Under these conditions, the yield of polysaccharides was 2.83%.关键词
石榴籽/多糖/复合酶法/D-最优混料设计/响应面分析法/提取工艺Key words
pomegranateseeds/polysaccharide/enzymaticextractionwithmixedenzymes/D-optimalmixturedesign/response surface analysis/extraction process分类
医药卫生引用本文复制引用
王占一,张立华,王玉海,戴博,郑丹丹,王飞..复合酶法提取石榴籽多糖的工艺优化[J].食品科学,2016,37(18):19-25,7.基金项目
2012年度国家星火计划项目（2012GA740049）；山东省自然科学基金项目 （）
