食品科学2016，Vol.37Issue(18)：26-33,8.DOI:10.7506/spkx1002-6630-201618005
响应面试验优化超声波辅助提取山杏种皮黑色素工艺及其抗氧化活性
Response Surface Optimization of Ultrasonic-Assisted Extraction of Melanin from Testae of Wild Apricots and Its Antioxidant Activities
摘要
Abstract
In this work, we isolated melanin from the testae of wild apricots (MTWA) by ultrasonic-assisted extraction. The optimization of the extraction process was carried out using by the combined use of one-factor-at-a-time method and response surface analysis (RSA) with Box-Behnken experimental design. Then the antioxidant activities of MTWA were analyzed including free radical scavenging capacity, metal ion-chelating capacity and lipid peroxidation inhibition. The results showed that the optimal extraction conditions were determined as follows: NaOH concentration, 0.33 mol/L; temperature, 63.07℃;time,3 h; and solid-to-solvent ratio, 1:10 (g/mL). Under these conditions, the maximum yield of MTWA of 4.14% was obtained. The in vitro antioxidant tests showed that MTWA had weaker 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate),radicalscavenging activities and ferric reducing ability. However, compared with synthetic melanin, MTWA had much stronger metal ion-chelating ability. MTWA also had stronger hydroxyl radical-scavenging activity and provided significant inhibition of lipid peroxidation induced by H2O2 and Fe2+-VC.关键词
超声波辅助提取/山杏种皮/黑色素/响应面法/抗氧化Key words
ultrasnic-assisted extraction/wild apricot testae/melanin/response surface analysis/antioxidant activities分类
农业科技引用本文复制引用
李红姣,李巨秀,赵忠..响应面试验优化超声波辅助提取山杏种皮黑色素工艺及其抗氧化活性[J].食品科学,2016,37(18):26-33,8.基金项目
国家林业公益性行业科研专项 （）
