食品科学2016，Vol.37Issue(18)：71-76,6.DOI:10.7506/spkx1002-6630-201618012
半制备型高效液相色谱法分离刺葡萄花色苷单体
Preparation of Monomeric Anthocyanins fromVitis davidii Foex by Semi-Preparative HPLC
摘要
Abstract
A semi-preparative high performance liquid chromatographic (HPLC) method using an XCharge C18 preparative column (20 mm× 250 mm, 10μm) was established for the preparation of anthocyanins monomers with high purity from crude extracts of Vitis davidiiFoex after purification with macroporous resin HP-20. The effects of experimental conditions including mobile phase gradient elution conditions, flow rate and injection volume were investigated on the separation efficiency of anthocyanins. The optimal chromatographic conditions were determined as follows: mobile phase, a mixture of methanol and 3% formic acid water for gradient elution; flow rate, 15 mL/min; and injection volume, 1.2 mL, which allowed the isolation and preparation of two major anthocyanins monomers in 18 min. The two anthocyanins were identified by MSE method using ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF MSE) as malvidin-3,5-O- diglucoside and malvidin-3,5-O-diglucoside-coumary with purities of 99.54% and 98.28%, respectively. The developed procedure was simple, effective, scalable and economic. It could be a promising alternative procedure for large-scale preparation of standard materials of anthocyanins fromVitis davidiiFoex.关键词
刺葡萄/锦葵素/半制备液相色谱/超高效液相色谱-四极杆飞行时间质谱Key words
Vitis davidiiFoex/malvidin/semi-preparative HPLC/UPLC-QTOF MSE分类
轻工纺织引用本文复制引用
王维茜,邓洁红,刘永红..半制备型高效液相色谱法分离刺葡萄花色苷单体[J].食品科学,2016,37(18):71-76,6.基金项目
国家自然科学基金面上项目（31271836）；湖南省教育厅研究生科研创新课题 （）
