食品科学2016，Vol.37Issue(18)：216-222,7.DOI:10.7506/spkx1002-6630-201618035
不同采收贮藏条件下鸭梨果实LOX基因表达及其与果心褐变的关系
LOX Gene Expression and Its Relationship with Core Browning of Yali Pear under Different Harvest and Storage Conditions
摘要
Abstract
Hebei-grown Yali pears (Pyrus bretschneideri Rehd cv. Yali) of different maturities were stored at (0± 1)℃directly or after pre-cooling at gradually decreasing temperature. Changes in respiration intensity, ethylene production and core browning index and lipoxygenase (LOX) activity andLOX gene expression pattern during storage were determined and the relationship ofLOX gene expression with core browning was studied as well. The results showed that during storage, the decreasing order of LOX activity and core-browning index was as follows: late-harvested fruit > early-harvested fruit > middle-harvested fruit, as well as rapid-cooling fruit > slow-cooling fruit. The expression level ofLOX gene in core tissues initially increased slowly, then declined, and increased again until the end of storage. The expression level ofLOX gene in rapid-cooling fruits was higher than in slow-cooling fruits, and the expression level ofLOX gene in middle maturity fruits was lower than in fruits of early and late maturities. Middle-maturity harvest combined with slow pre-cooling inhibited the LOX gene expression of flesh and core tissues, thereby suppressing core browning of Yali pears. LOX activity and the gene expression level ofLOX were coordinated with core browning index, suggesting that the expression level of this gene was correlated closely with core browning of Yali pears.关键词
鸭梨/脂氧合酶/果心褐变/基因表达/褐变指数Key words
Yali pear/lipoxygenase/core browning/gene expression/core browning index分类
农业科技引用本文复制引用
韩云云,宋方圆,韩艳文,李玲,闫师杰..不同采收贮藏条件下鸭梨果实LOX基因表达及其与果心褐变的关系[J].食品科学,2016,37(18):216-222,7.基金项目
国家自然科学基金面上项目 （）
