沈阳农业大学学报2016，Vol.47Issue(4)：438-444,7.DOI:10.3969/j.issn.1000-1700.2016.04.009
一例副猪嗜血杆菌的分离鉴定及16S rRNA生物信息学分析
Isolation and Identification for One Case of Haemophilus parasuis and Bioinformatic Analysis for Its 16S rRNA Gene
摘要
Abstract
At a large-scale pig farm in Shenyang, a strain of polymorphic Gram-negative bacteria was isolated from the lungs of sick pigs suspected with infected by Haemophilus parasuis. According to the culture characteristics and biochemical test results, as well as the PCR identification and sequence alignment of Haemophilus parasuis 16s rRNA specific primer, the homology between the isolate and Hps in GenBank was 99%. Therefore, the isolate is identified as Haemophilus parasuis. The results of antibiotic drug sensitivity test showed that the isolate was more sensitive to Carbenicillin, Ampicillin, Cefazolin, Caftriaxone, Cefoperazone and resistant to Kanamycin, Co-trimoxazole, Ceftazidime and Tetracycline. The genetic evolutionary relationship of 16s rRNA displayed that the isolate was on the same branch with 16s rRNA sequence of Haemophilus parasuis strain 2 of serotype 5, with a close genetic evolutionary relationship between them, and the Nucleotide homology was between 97.4%-100%. Thus it was identified preliminarily as serotype 5 Haemophilus parasuis and named the SY-1 strain. The results provided a basis for diagnosis and clinical prevention and control of the Haemophilus parasuis disease.关键词
副猪嗜血杆菌/分离鉴定/药敏试验/16S rRNA/序列分析Key words
Haemophilus parasuis/isolation and identification/drug sensitivity test/16S rRNA/sequence analysis分类
农业科技引用本文复制引用
李曦婷,尹荣焕,王超,薛彦杰,李化生,张翔,尹荣兰,董俏,刘梦志,李睿..一例副猪嗜血杆菌的分离鉴定及16S rRNA生物信息学分析[J].沈阳农业大学学报,2016,47(4):438-444,7.基金项目
辽宁省教育厅优秀人才支持项目(LJQ2013070) （LJQ2013070）
辽宁省自然科学基金项目(201602667) （201602667）
