茶叶科学2016，Vol.36Issue(5)：461-468,8.
茶多酚对直链烷基苯磺酸钠致小鼠皮肤损伤的保护作用
The Protective Effects of Tea Polyphenol on Injury of Skin Tissue of Mice Induced by Linear Alkylbenzene Sulfonate
摘要
Abstract
The objective of the study is to investigate the protective effects of tea polyphenol (TP) on injury of skin tissue of mice induced by linear alkylbenzene sulfonate (LAS). Fifty healthy Kunming mice (male) were randomly divided into 5 groups including normal control group (NC), TP control group (TPC), LAS-injury group (LAS, 300 mg·L-1), TP-intervention group 1 (LAS 300 mg·L-1, TP 100 mg·L-1) and TP-intervention group 2 (LAS 300 mg·L-1, TP 200 mg·L-1) respectively (n=10). The skins on the back of all mice were shaved and treated with distilled water and different concentrations of LAS and TP for 60 days. The observational indexes included general condition of mice , HE and Masson staining of skin, the levels of malondialdehyde (MDA), super oxidase dimutase (SOD), glutathione peroxidase (GSH-Px), hydroxyproline (Hyp), lactate dehydrogenase (LDH) and nitric oxide (NO) in skin and serum. The results showed that administration of TP to LAS-treated mice inhibited the oxidative stress and destruction of collagen fiber of skin induced by LAS. Compared with LAS-injury group, the body weight and skin thickness of mice of TP-intervention groups were obviously increased after treatment for 6 weeks (P<0.01). Additionally, the contents of collagen fibers were increased and the structure of collagen fibers was improved. Moreover, the levels of SOD, GSH-Px and Hyp were significantly increased, but the levels of MDA, LDH and NO were remarkably reduced (P<0.01). These results suggest that TP can protect skin of mice from LAS-induced injury.关键词
茶多酚/直链烷基苯磺酸钠/皮肤组织/损伤/保护作用Key words
tea polyphenol/linear alkylbenzene sulfonate/skin tissue/injury/protection分类
农业科技引用本文复制引用
赵文红,崔慧娴,孙倩男,陈兵兵..茶多酚对直链烷基苯磺酸钠致小鼠皮肤损伤的保护作用[J].茶叶科学,2016,36(5):461-468,8.基金项目
安徽省高等学校自然科学重点研究项目（KJ2014A159）、安徽省高等学校省级质量工程教学研究项目（2014jyxm210）、安徽省高等学校省级质量工程特色专业建设项目（2014tszy019）。 （）
