茶叶科学2016，Vol.36Issue(5)：469-476,8.
L-茶氨酸对产肠毒素大肠杆菌引起的免疫应激小鼠肠道的保护作用研究
Study on the Protective Effect of L-Theanine on the Intestinal in Mice with Immune Stress Induced by ETEC
摘要
Abstract
SPF grade Balb/c female mice were used as experimental animals. After adaptive feeding for 3 days, mice were orally pre-treated with different dosages of L-theanine once daily for 30 days before ETEC E44813 intraperitoneal injection. The body weight change in mice was analyzed during the breeding process. Mice were scarified 5 hours after ETEC injection and tissues were collected for analysis. The activities of ileum Glutathione peroxidase (GSH-Px), homogenate Catalase (CAT), Malondialdehyde (MDA) and Inducible nitric oxide synthase (iNOS) were measured and the pathological changes of intestinal and the levels of serum Tumor necrosis factor-α(TNF-α), Interleukin-1β (IL-1β) and Intercellular cell adhesion molecule-1(ICAM-1) were examined. This study was aimed to explore the protective effects of L-theanine on intestinal of ETEC-induced immune stress model in mice. The results showed that: pretreatment with L-theanine at different dosages significantly increased mouse body weight;lowered the levels of ileum homogenate MDA and iNOS, improved the activities of ileum homogenate CAT, GSH-Px, decreased the expressions of serum TNF-α、IL-1β and ICAM-1, and improved pathological structure of jejunum tissue. These data demonstrated that pre-treatment with L-theanine prevented ETEC-induced intestinal injury through inhibiting TNF-α、IL-1β and ICAM-1, and inflammatory reaction and enhancing intestinal antioxidant abilities.关键词
L-茶氨酸/产肠毒素大肠杆菌/免疫应激/保护肠道Key words
L-theanine/Enterotoxigenic Escherichia coli./immune stress/intestinal protection分类
生物科学引用本文复制引用
刘遵莹,刘秋玲,邓燕莉,陈凌,肖文军..L-茶氨酸对产肠毒素大肠杆菌引起的免疫应激小鼠肠道的保护作用研究[J].茶叶科学,2016,36(5):469-476,8.基金项目
国家“十二五”科技支撑计划项目 （）
