茶叶科学2016，Vol.36Issue(5)：477-483,7.
茶多糖对阿霉素抑制肺癌 A549细胞增殖作用的影响
Tea Polysaccharide Increased Doxorubicin Inhibition of Lung Cancer A549 Cells
魏楠 1朱强强 2陈际名 1李彤 2李亦凡 3黄业伟 1马啸 2王宣军 3盛军1
作者信息
- 1. 云南农业大学普洱茶学教育部重点实验室，云南 昆明 650201
- 2. 云南农业大学龙润普洱茶学院云南省茶深加工工程技术研究中心，云南 昆明 650201
- 3. 云南农业大学食品科学技术学院，云南 昆明 650201
- 折叠
摘要
Abstract
Lung cancer has high incidence and mortality rates around the world and chemotherapy is widely applied in cancer treatment. Doxorubicin (DOX) is a kind of chemotherapy drug which commonly used in clinical trials. However, the drug has many side effects and may cause a dose-dependent injury in the long term. These side effects include irreversible cardiopulmonary, bone marrow suppression and so on. Moreover, its mufti-drug resistance also restricts it in certain clinical application. The effect of polysaccharides of tea extract combined with doxorubicin on human lung cancer cell line A549 were tested in this study. A549 was first cultured in vitro with different concentrations of DOX. After 24h culture, apotheosis ratio was determined by MTT. The results show that the DOX dose for the best inhibiting effect was 3 mg·L-1 according to MTT detection. Three doses of DOX were selected to combine tea polysaccharide and suppress A549 cell survival. The combination of 2 mg·L-1 DOX and 6 mg·L-1 tea polysaccharide had been demonstrated to have better suppressing efficiency than 3 mg·L-1 DOX alone. DOX could efficiently suppress A549 cell proliferation and this inhibiting effect was enhanced by combining with tea polysaccharide, which could decrease the applied dose of DOX.关键词
茶多糖/阿霉素/抗肿瘤Key words
tea polysaccharides/doxorubicin/antitumor分类
生物科学引用本文复制引用
魏楠,朱强强,陈际名,李彤,李亦凡,黄业伟,马啸,王宣军,盛军..茶多糖对阿霉素抑制肺癌 A549细胞增殖作用的影响[J].茶叶科学,2016,36(5):477-483,7.
