茶叶科学2016，Vol.36Issue(5)：484-490,7.
L-茶氨酸对产肠毒性大肠杆菌引起的免疫应激小鼠肝脏的保护作用研究
Study on the Protective Effect of L-Theanine on the Liver of Mice with Immune Stress Induced by ETEC
摘要
Abstract
SPF grade Balb/c female mice were used for this experiment. After adaptive feeding for 3 d, the mice were pre-treated orally with different dosages of L-theanine once daily for 30 d. ETEC E44813 intraperitoneal injection was subsequently performed and samples were collected 5h later. The coefficient of liver and spleen, levels of serum alanine aminotranferease (ALT), aspartate aminotransferase (AST) as well as liver homogenate malondialdehyde (MDA), and activities of liver homogenate superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), pathological changes of liver, expressions of serum γ-Interferon (IFN-γ) and Interleukin-4 (IL-4) were studied to explore the protective effects of L-theanine on liver of ETEC immune mouse model. Results show that pretreatment with L-theanine significantly reduced the increase of liver and spleen coefficient caused by ETEC infection, lowered the levels of serum ALT, AST and liver homogenate MDA, increased the activities of liver homogenate SOD, CAT, GSH-Px, decreased the expressions of serum IFN-γ, IL-4, and improved pathological injury of liver tissue. Therefore, it can be concluded that L-theanine is able to prevent ETEC-induced hepatic injury through depressing IFN-γ, IL-4 and inflammatory reaction and enhancing hepatocyte antioxidant abilities.关键词
L-茶氨酸/产肠毒性大肠杆菌 E44813/免疫应激/肝脏保护Key words
L-theanine/enterotoxigenic Escherichia coli E44813/immune stress/liver protection分类
生物科学引用本文复制引用
刘秋玲,龚志华,陈凌,刘遵莹,邓燕莉,陈栋,肖文军..L-茶氨酸对产肠毒性大肠杆菌引起的免疫应激小鼠肝脏的保护作用研究[J].茶叶科学,2016,36(5):484-490,7.基金项目
国家“十二五”科技支撑计划项目 （）
