茶叶科学2016，Vol.36Issue(5)：505-512,8.
茶树硝态氮转运蛋白 NRT1.1基因的克隆及表达分析
Cloning and Expression Analysis of Nitrate Transporter NRT1.1 Gene in Tea Plant (Camellia sinensis (L.))
摘要
Abstract
A full length cDNA sequence of Nitrate transport gene (NRT1.1) was obtained from tea plant (Camellia sinensis (L.)) cultivar ‘Longjing 43’ by polymerase chain reaction (PCR) and rapid amplification of cDNA ends PCR (RACE-PCR). The length of nucleotide sequence of this gene was 1 880 bp, containing a complete open reading frame (1 788 bp) to encode 595 amino acids. The putative protein had an isoelectric point of 8.99 and a calculated molecular weight of 65.9 kD. CsNRT1.1 was highly homologous to the gene NRT1.1 in Vitis vinifera by sequence alignment. Several parameters of these sequences, including sequences composition, physicochemical property, topological structure of transmembrane regions, hydrophobicity or hydrophilicity, subcellular localization were predicted by bioinformatics tools. Quantitative real-time PCR analysis showed that the expression of CsNRT1.1 in roots and leaves were inhibited after incubation in 1 mol·L-1 NO3- for 5 min. The expressions of CsNRT1.1 in roots were always lower than that of CK within 24 h. Its expressions in leaves were higher than those in roots with its peak at 0.5 h. Our results provides favorably help to reveal NO3- uptake and utilization in tea plants.关键词
茶树/硝态氮/NRT1.1 基因/实时定量 PCRKey words
Camellia sinensis/nitrate/NRT1.1/RT-PCR分类
农业科技引用本文复制引用
杨亦扬,胡雲飞,万青,李荣林,王枫,阮建云..茶树硝态氮转运蛋白 NRT1.1基因的克隆及表达分析[J].茶叶科学,2016,36(5):505-512,8.基金项目
国家自然科学基金（31400587）、江苏省自然科学基金（BK20160590）、茶树生物学与资源利用国家重点实验室开放基金（SKLTOF20150114）、江苏省农业科技自主创新资金（CX(16)1003）。 （16）
