食品科学技术学报2016，Vol.34Issue(5)：26-32,7.DOI:10.3969/j.issn.2095-6002.2016.05.004
碱性蛋白酶水解螺旋藻制备抗氧化活性肽的工艺优化
Optimization of Hydrolysis Conditions for Antioxidative Peptides from Spirulina Using Alcalase
摘要
Abstract
The hydrolysis conditions of antioxidant peptides from Spirulina powder using alcalase were op-timized by single factor methods and the Box-Behnken experiments, in order to improve the antioxidant abilities and the yield of Spirulina peptides. The results showed that alcalase has the highest hydrolysis ability compared with other commercial proteases, incluing papain, trypsase and flavourzyme, and it’s op-timum hydrolysis conditions were 55. 46℃, pH 6. 71 and the solid liquid ratio was 1:10. 83 . When the Spirulina powder were hydrolyzed for 240 min under the optimized conditions, the yield of the Spirulina peptides was 58. 50%, increasing by 15. 61% compared with the control. The DPPH radical scavenging ability of Spirulina peptides reached 77. 597%, at the mass fraction of Spirulina peptides was 0. 86 g/L, increased by 7. 62% compared with the control.关键词
螺旋藻/碱性蛋白酶/抗氧化活性肽/DPPH·清除能力Key words
Spirulina/alcalase/antioxidant peptides/DPPH radical scavenging ability分类
轻工纺织引用本文复制引用
马艳芳,李玉洋,刘金龙,郑明刚,孙中涛..碱性蛋白酶水解螺旋藻制备抗氧化活性肽的工艺优化[J].食品科学技术学报,2016,34(5):26-32,7.基金项目
国家海洋局海洋生物活性物质与现代分析技术重点实验室开放基金资助项目(MBSMAT-2014-05)。 （MBSMAT-2014-05）
