食品科学技术学报2016，Vol.34Issue(5)：43-47,5.DOI:10.3969/j.issn.2095-6002.2016.05.006
4种培养基对红曲霉M1莫纳可林K产量影响及基因差异表达分析
Effect of Four Kind of Medium on Monascus M1 with Monacolin K Production and Real-time Quantitative Analysis
摘要
Abstract
Monacolin K is one of the important secondary metabolites of Monascus sp. The influence of Monacolin K production for Monascus M1 on four kinds of fermentation substrate were studied. And the mRNA expression of Monacolin K biosynthesis gene cluster was measured by RT-qPCR. The result showed that the concentration of Monacolin K was the highest(8. 49 mg/L) cultured by buckwheat while the concentration of Monacolin K was 7. 12, 4. 77, 2. 11 mg/L cultured by brown rice, oats, and rice. And there was positive correlation between the expression genes related to MK and the MK production, except for MKB and MKC. The different kinds of culture medium could influence the secondary metabo-lites production by stimulating the gene clusters.关键词
红曲霉/莫纳可林K/培养基/RT-qPCRKey words
Monascus sp./Monacolin K/culture medium/RT-qPCR分类
轻工纺织引用本文复制引用
林琳,李贞景,陈勉华,高雅娟,武慧佳,王昌禄..4种培养基对红曲霉M1莫纳可林K产量影响及基因差异表达分析[J].食品科学技术学报,2016,34(5):43-47,5.基金项目
国家自然科学基金资助项目(31171729)。 （31171729）
