食品科学技术学报2016，Vol.34Issue(5)：48-54,7.DOI:10.3969/j.issn.2095-6002.2016.05.007
酸性木聚糖酶交联酶聚集体的制备条件优化
Optimizing Preparation Conditions of Cross-linked Acid-stable Xylanase Aggregates
摘要
Abstract
The cross-linked enzyme aggregates method was a new carrier-free immobilized enzyme tech-nology. In order to improve the stability of xylanase, the technology was applied for the preparation of the acid-stable xylanase from Penicillium janthinellum. This study explored conditions of the preparation of cross-linked xylanase aggregates. The results showed that the crude xylanase at the concentration of 0. 36 mg/mL was precipitated with 85% saturated ammonium sulfate for 30 min at 40℃. Then the aggregates were cross-linked by 0. 14% glutaraldehyde solution for 4 h at 40 ℃. The obtained activity of cross-linked xylanase aggregates remained 42. 2% of total enzyme activity, which is helpful for the further ap-plication of acid xylanase in the industry.关键词
微紫青霉/酸性木聚糖酶/交联酶聚集体/无载体固定化Key words
Penicillium janthinellum/acid-stable xylanase/cross-linked enzyme aggregates/carrier-free immobilized分类
轻工纺织引用本文复制引用
吴春玲,李璠,马超,袁月,范光森,李秀婷..酸性木聚糖酶交联酶聚集体的制备条件优化[J].食品科学技术学报,2016,34(5):48-54,7.基金项目
国家自然科学基金面上项目(31371723) （31371723）
北京市自然科学基金青年项目(6164029)。 （6164029）
