食品科学2016，Vol.37Issue(19)：31-35,5.DOI:10.7506/spkx1002-6630-201619005
磁性Fe3O4纳米带鱼肽微粒的制备及对CW-2细胞膜流动性的影响
Preparation of Magnetic Fe3O4 Nanoparticles Loading Peptides Derived from Enzymatic Hydrolysis of Hairtail Waste Proteins and Its Effect on Membrane Fluidity in CW-2 Cells
摘要
Abstract
Objective: To prepare magnetic Fe3O4 nanoparticles loading peptides derived from enzymatic hydrolysis of hairtail processing waste proteins and explore its effect on membrane fluidity in CW-2 cells. Methods: The target product was synthesized by co-precipitation method, and its structure characteristics and particle size distribution were observed with X-ray diffraction (XRD), transmission electron microscope (TEM) and atomic force microscope (AFM). Meanwhile, its effect on membrane fluidity in human colon cancer CW-2 cels was explored by fluorescence polarization in a non-magnetic field and an alternating magnetic field. Results: Magnetic Fe3O4 nanoparticles loading hairtail peptides were uniformly distributed with a size of approximately 10 nm and they were found to adhere to each other to form intertwined curves. Compared with the single magnetic Fe3O4 particles, loading of hairtail peptides enhanced dispersion stability of magnetic nano-Fe3O4 particles. The optimum pH for magnetic Fe3O4 nanoparticles loading hairtail peptides was 6.5–9.0. Cel membrane fluidity tests showed thatP andη values in the experimental group were significantly reduced after 24 h of alternating magnetic field exposure, indicating that the magnetic Fe3O4 nanoparticles loading hairtail peptides could increase cel membrane fluidity. Conclusion: Magnetic Fe3O4 nanoparticles loading hairtail peptides can enhance the anti-tumor activity of hairtail peptides in an alternating magnetic field.关键词
磁性Fe3O4纳米微粒/带鱼酶解小肽/结构表征/CW-2细胞/细胞膜流动性Key words
magnetic Fe3O4 nanoparticles/hairtail enzymatic peptides/structure characterization/CW-2 cel/cel membrane fluidity分类
农业科技引用本文复制引用
石芸洁,林慧敏,金图南,邓尚贵..磁性Fe3O4纳米带鱼肽微粒的制备及对CW-2细胞膜流动性的影响[J].食品科学,2016,37(19):31-35,5.基金项目
国家自然科学基金青年科学基金项目（31301597）；国家自然科学基金面上项目（31471609）；海洋公益性行业科研专项 （）
