食品科学2016，Vol.37Issue(19)：59-63,5.DOI:10.7506/spkx1002-6630-201619010
氨基酸修饰可食用生物膜载体的制备与性质
Preparation and Properties of Edible Amino Acid-Modified Biofilm Carrier
摘要
Abstract
In order to expand the application of lactic acid bacterial biofilm in the food industry and construct edible functional carrier, amino acids which could be used as ligands for bacterial adhesion were modified onto alginate in this study. The structures, compositions, electrical properties and hydrophilicity of the modified carriers (Alg-Arg and Alg-Lys) were characterized by nuclear magnetic resonance, amino acid content analysis, and ζ potential and water contact angle measurement.Lactobacillus kefiranofaciensZW3 was cultured on the carriers to investigate its growth and extracellular exopolysaccharide secretion. The results showed that after cultivation for 4 d, the amount of ZW3 on Alg-Arg reached up to 3.8 × 107 CFU/cm2, which was 3.5 times as high as that on Alg-Lys. The secretion amount of extracellular exopolysaccharide was 28.2 μg/cm2, which was increased by 2.4 times in comparison with that on Alg-Lys. Compared with lysine-modified carrier, arginine-modified carrier was better for ZW3 growth and the secretion of extracellular exopolysaccharide. These results demonstrated that biofilm formation could be enhanced by the appropriate ligand.关键词
氨基酸/海藻酸钠/生物膜/乳酸菌/配体Key words
amino acid/sodium alginate/biofilm/Lactobacillus/ligand分类
轻工纺织引用本文复制引用
孙也,王艳萍,李淑雅,刘兆贤,刘媛..氨基酸修饰可食用生物膜载体的制备与性质[J].食品科学,2016,37(19):59-63,5.基金项目
国家高技术研究发展计划（863计划）项目（2011AA10090405）；天津科技大学大学生实验室创新基金项目（1414A208）；天津科技大学青年创新基金项目 （）
