食品科学2016，Vol.37Issue(19)：123-127,5.DOI:10.7506/spkx1002-6630-201619021
对羟基苯甲醇对灰树花产胞外多糖的影响及其发酵动力学
Effect ofp-Hydroxybenzyl Alcohol on the Biosynthesis of Exopolysaccharide by Submerged Culture of Grifola frondosaand Fermentation Kinetics
摘要
Abstract
The effect of adding different concentration gradients ofp-hydroxybenzyl alcohol to the culture medium ofGrifola frondosa on mycelial growth and the production of extracellular polysaccharide in submerged fermentation. Meanwhile, we determined changes in mycelial growth, reducing sugar (residual sugar) content, extracellular polysaccharide content, pH, p-hydroxybenzyl alcohol and gastrodin contents in the fermentation broth ofGrifola frondosa, and we also made a dynamic analysis. Results showed that addition of 200 mg/Lp-hydroxybenzyl alcohol was found to be optimal, resulting in an increase in mycelial biomass of 22.73% and an increase in extracellular polysaccharide production of 10.24% as compared with the control group without addedp-hydroxybenzyl alcohol; both increases were significant (P < 0.05). Dynamic analysis results showed that the growth ofGrifola frondosa remained stable from day 8 onwards; glucose was consumed as the carbon source, and residual glucose and the production of extracellular polysaccharide both remained stable from day 10 onwards. In addition, the gastrodin content increased as a result of partial conversion ofp-hydroxybenzyl alcohol into gastrodin.关键词
对羟基苯甲醇/灰树花/胞外多糖/生物量/发酵动力学Key words
p-hydroxybenzyl alcohol/Grifola frondosa/extracellular polysaccharide/biomass/fermentation kinetics分类
生物科学引用本文复制引用
朱俊杰,吴天祥,吴彩云,刘昕,赵群丽..对羟基苯甲醇对灰树花产胞外多糖的影响及其发酵动力学[J].食品科学,2016,37(19):123-127,5.基金项目
国家自然科学基金地区科学基金项目 （）
