食品科学2016，Vol.37Issue(19)：166-170,5.DOI:10.7506/spkx1002-6630-201619028
食源性沙门氏菌耐药表型与耐药基因的研究
Evaluation of Drug-Resistant Phenotypes and Genes in FoodborneSalmonella Isolates
摘要
Abstract
In order to investigate the relationship between drug-resistant phenotypes and genes and explore the mechanism of drug resistance at the gene level, a total of 215Salmonella strains isolated from 1 093 retail meat samples were assayed for antimicrobial susceptibility. Totally 16 pairs of primers were designed to amplify the drug-resistant genes and sequence homology was detected after T-A cloning. Results indicated that theSalmonella isolates showed the highest resistance to sulfisoxazole (SIZ) (71.7%), followed by tetracycline (TET) (69.8%), trimethoprim (TMP) (67.9%) and compound sulfamethoxazole (TSU) (52.8%), and all these isolates were sensitive to ciprofloxacin (CIP), nitrofurantoin (NIT) and cephalosporin. Approximately 60.5% of these isolates showed resistance to at least 3 kinds of antibiotics, and 4 of them were even resistant to 13 drugs. Ten drug-resistant genes were detected and they were found to share a homology higher than 99% with reference sequences. The consistency rates between drug-resistant phenotype and drug-resistant gene forβ-lactams and tetracycline were both higher than 90%, followed by sulfonamides (81.6%). This study demonstrated that the drug resistance rate of foodborne Salmonella was high and their multi-drug resistance was serious. And the results of detection of drug-resistant phenotypes and drug-resistant genes were consistent.关键词
食品安全/沙门氏菌/抗生素/耐药表型/耐药基因Key words
food safety/Salmonella/antibiotics/antimicrobial resistance phenotype/resistance genes分类
轻工纺织引用本文复制引用
侯雪娇,吴科敏,莫国东,刘书宏,言天久,焦鹏涛,韦平..食源性沙门氏菌耐药表型与耐药基因的研究[J].食品科学,2016,37(19):166-170,5.基金项目
广西科学研究与技术开发计划项目（桂科重14121003-4-1）；国家现代农业（广西肉鸡）产业技术体系创新团队建设专项 （）
