食品科学2016，Vol.37Issue(19)：237-242,6.DOI:10.7506/spkx1002-6630-201619040
光棘球海胆多肽的制备及其生物活性
Preparation and Bioactivity of Peptides from Strongylocentrotus nudus
摘要
Abstract
Strongylocentrotus nudus is one of the major species of economic sea urchin in China. It has high food and medicinal value due to its delicious taste and nutritional richness. In this work, bioactive peptides were prepared by enzymatic hydrolysis ofStrongylocentrotus nudus gonads with papain and their bioactivities were investigated. Five fractions with molecular weight distribution of < 1, 1–3, 3–5, 5–10 and <10 kD were obtained by ultrafiltration of the hydrolysate. Results showed that all the peptide fractions at a final concentration of 200g/mL could promote lymphocyte growth. Moreover, at this concentration, all the peptides except the fraction of < 1 kD could inhibit the growth of HeLa cells, and promote the phagocytic function of peritoneal macrophages. At 50g/mL, all the peptide fractions could protect Chang liver cells against oxidation induced by tert-butyl hydroperoxide;butylhydroperoxid through direct and indirect pathways. All the peptides could inhibit the classical complement pathway activity in a dose-dependent manner. All the above results suggested potential regulatory activity on cellular and humoral immune function and anti-oxidant capacity. The multiple bioactivities of these peptides deserve further investigation.关键词
光棘球海胆/多肽/生物活性/人宫颈癌细胞Key words
sea urchin/peptide/bioactivities/HeLa cell分类
农业科技引用本文复制引用
王婷,李冬梅,张公亮,侯红漫,孙黎明,王晓慧,马莹,季晓彤,王玲,年益莹,薛鹏,孟双,秦磊..光棘球海胆多肽的制备及其生物活性[J].食品科学,2016,37(19):237-242,6.基金项目
辽宁省自然科学基金计划项目（2015020794）；辽宁省优秀人才项目 （）
