食品科学2016，Vol.37Issue(20)：6-12,7.DOI:10.7506/spkx1002-6630-201620002
响应面试验优化高温高压制备玉米环（组氨酸-脯氨酸）二肽关键工艺
Optimization of Key Processing Parameters for Preparation of Corn Cyclo (His-Pro) by High Temperature/High Pressure Method
摘要
Abstract
In this study, we investigated the synthesis of corn cyclo (His-Pro) (CHP) in aqueous solution by a high temperature/pressure method from corn protein hydrolysate prepared in our laboratory. First of al , the one-factor-at-a-time method was used to investigate the influences of reaction temperature, substrate concentration, KHCO3 concentration and reaction time on CHP yield. Subsequently, the reaction conditions were optimized using response surface methodology. Results revealed that the optimum reaction conditions were determined as follows: reaction temperature, 125.9 ℃; reaction pressure, 0.25 MPa; substrate concentration, 20.5 mg/mL; KHCO3 concentration, 0.16 mol/L; and reaction time, 5.3 h, resulting in the maximum yield of CHP as high as 6.58 mg/g. The prepared CHP exhibited the expected structure as confirmed by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).关键词
玉米蛋白/环(组氨酸-脯氨酸)二肽/高温高压法/环化反应Key words
corn protein/cyclo (His-Pro)/high temperature/high pressure method/cyclization reaction分类
轻工纺织引用本文复制引用
樊红秀,刘婷婷,刘鸿铖,任华华,张艳荣..响应面试验优化高温高压制备玉米环（组氨酸-脯氨酸）二肽关键工艺[J].食品科学,2016,37(20):6-12,7.基金项目
“十二五”国家科技支撑计划项目（2013BAD16B08）；国家高技术研究发展计划（863计划）项目 （）
