食品科学2016，Vol.37Issue(20)：19-23,5.DOI:10.7506/spkx1002-6630-201620004
贵长猕猴桃多糖提取工艺及体外抗氧化功能
Extraction and Antioxidant Activity of Polysaccharides from Guichang Kiwifruit
摘要
Abstract
The extraction and antioxidant activity of polysaccharides from Guichang kiwifruit pulp or pericarp were investigated in this work. The optimization of the extraction parameters was investigated by using orthogonal array design. Polysaccharide content was determined by the anthranone-sulfuric acid method. The antioxidant activities of Guichang kiwifruit polysaccharides were tested by pyrogallol autoxidation and Fenton’s oxidation reaction. The results showed that the optimum extraction conditions for kiwifruit pulp polysaccharides were found to be 2 h extraction at 80℃ with a solid-to-solvent ratio of 1:35 (g/mL) performed three times, and those for kiwifruit pericarp polysaccharides were determined as follows: three extraction cycles at 70℃ each for 2 h at a solid-to-solvent ratio of 1:25 (g/mL) . Under the optimized conditions, the yield of polysaccharide extracted from kiwifruit pulp and pericarp reached 1.74% and 1.16%, respectively. The polysaccharides from kiwifruit pulp and pericarp both had strong scavenging effects on superoxide anion and hydroxyl radicals with half inhibitory concentration (IC50)of 12.4 and 41.3 μg/mL for pulp polysaccharides and 84.1 and 50.8 μg/mL for pericarp polysaccharides, respectively.关键词
贵长猕猴桃/果肉/果皮/多糖/提取工艺/抗氧化能力Key words
Guichang kiwifruit/pulp/pericarp/polysaccharide/extraction process/antioxidant activity分类
轻工纺织引用本文复制引用
王金华,杜超,梁晨,马立志,曾加..贵长猕猴桃多糖提取工艺及体外抗氧化功能[J].食品科学,2016,37(20):19-23,5.基金项目
贵州省科学技术基金项目（黔科合LH字[2014]7174号）；贵州省教育厅自然科学研究项目（黔教合KY字[2013]199号；黔教合KY字[2014]309号）；贵州省普通高等学校创新人才团队建设项目（黔教合人才团队字[2014]44号）；食品科学与工程重点学科项目（黔学位合字ZDXK[2014]13号）；贵州省果品加工、贮藏与安全控制协同创新中心项目 （）
