食品科学2016，Vol.37Issue(20)：37-41,5.DOI:10.7506/spkx1002-6630-201620007
离子液体辅助超声萃取-高效液相色谱同时测定半边莲中6种黄酮类化合物
Simultaneous Determination of 6 Flavonoid Compounds in Herba Lobelia chinensisby High Performance Liquid Chromatography after Ionic Liquid Assisted Ultrasonic Extraction
摘要
Abstract
Objectives: To optimize the conditions for ionic liquid-assisted extraction of lfavonoids compounds from Herba Lobelia chinensis using single factor and orthogonal array designs and consequently to establish a high performance liquid chromatography (HPLC) method for simultaneous determination of rutin, quercetin, naringgenin, hesperetin, kaempferol and apigenin in Herba Lobelia chinensis.Methods: The 6 compounds were separated on an InertSustain C18 (150 mm × 4.6 mm, 5 μm) using a mobile phase consisting of methanol and acetic acid (pH 3.0) with gradient elution at a lfow rate of 1.0 mL/min. The detection wavelength was set at 285 nm. The column temperature was maintained at 35℃. Results: The optimum extraction conditions for total lfavonoids were determined as follows: 80% (V/V) aqueous methanol as extraction solvent; solid-to-liquid ratio, 1:80 (g/mL); extraction time, 30 min; and ionic liquid concentration, 0.6 mol//L. Under these conditions, rutin, quercetin, naringgenin, hesperetin, kaempferol and apigenin were separated satisfactorily. The limits of detection (LODs) for these lfavonoid compounds were 0.001 2, 0.002 3, 0.002 6, 0.000 2, 0.001 0 and 0.000 6 μg/mL, respectively. The recoveries of spiked samples ranged from 91.77% to 102.53%. Conclusion: The proposed method is simple and rapid, and it can provide an efifcient means for the extraction, separation and determination of lfavonoid compounds in HerbaLobelia chinensis.关键词
高效液相色谱/半边莲/黄酮类化合物/离子液体/超声萃取Key words
high performance liquid chromatography/Herba Lobelia chinensis Lour./flavonoid compounds/ionic liquid/ultrasonic extraction分类
化学化工引用本文复制引用
邓永利,周光明,陈军华,高意,廖安辉..离子液体辅助超声萃取-高效液相色谱同时测定半边莲中6种黄酮类化合物[J].食品科学,2016,37(20):37-41,5.基金项目
中央高校基本科研业务费专项 （）
