食品科学2016，Vol.37Issue(20)：113-119,7.DOI:10.7506/spkx1002-6630-201620019
基于磁性免疫传感器的牛乳中四环素残留检测方法的构建
Establishment of Magnetic Bead-Based Immunosensor for the Detection of Tetracycline Residues in Milk
摘要
Abstract
A highly sensitive and rapid immunofluorescence assay for the detection of tetracycline residues in raw milk based on magnetic beads was developed. The limit of detection (LOD) and limit of quantiifcation (LOQ) of this method were 0.03 and 0.20 ng/mL, respectively. The lfuorescence intensity was linear with the concentration of tetracycline in the range of 0.25 to 32 ng/mL with a coefifcient of determination of 0.995 9 (R2= 0.995 9). The recoveries of TC spiked at different concentrations were between 82.6% and 97.0%, indicating that the method is accurate. Furthermore, precision, calculated as relative standard deviation (RSD), was below 5.0% for intra-day and inter-day determinations, indicating good repeatability of the method. The results of tetracycline residues in raw milk determined by this method and HPLC-UV were highly consistent.关键词
磁珠/免疫反应/荧光检测/四环素/生鲜乳Key words
magnetic beads/immunoreaction/fluorescence detection/tetracycline/raw milk分类
轻工纺织引用本文复制引用
康怀彬,马红燕,李芳,徐宝成,罗登林,张瑞华,邹良亮..基于磁性免疫传感器的牛乳中四环素残留检测方法的构建[J].食品科学,2016,37(20):113-119,7.基金项目
国家自然科学基金青年科学基金项目（31501563）；洛阳市畜牧局项目（2518）；河南科技大学校级创新团队项目 （）
