食品科学2016，Vol.37Issue(20)：177-182,6.DOI:10.7506/spkx1002-6630-201620030
实时荧光PCR法鉴定食品中双歧杆菌
Identification of Biifdobacterium Strains in Foods by Real-Time PCR
摘要
Abstract
A real-time PCR method was developed to identifyBiifdobacterium in foods. Speciifc primers and probes targeting the conserved region of the 16S rRNA gene ofBiifdobacteriumwere designed. The speciifcity, sensitivity, reproducibility and system anti-interference ability of the proposed method were veriifed. Twenty-ifve commercial products labeled “containing Bifidobacterium” were tested by the real-time fluorescence PCR assay. Results showed that the developed method was highly speciifc forBiifdobacterium detection. The detection limits were 2 pg using eight species ofBiifdobacterium as the templates, and the relative detection limits were 104CFU/mL. Standard deviations and relative standard deviations (RSDs) of Ct values for ifve DNA concentrations were all in the acceptable range. The Ct values were not affected by a mixture of Biifdobacterium adolescentis ATCC15703 andEscherichia coli ATCC25922 at both the culture level and genomic DNA level, which showed that the fluorescence PCR method has good anti-interference ability. Five samples were detected withoutBiifdobacterium. Thus, considering its high speciifcity, sensitivity and repeatability, the real-time PCR could be used to rapidly and accurately identifyBiifdobacterium in foods.关键词
实时荧光聚合酶链式反应/双歧杆菌/16S核糖体RNA/鉴定Key words
real-time polymerase chain reaction (RT-PCR)/Biifdobacterium/16S ribosomal RNA/identiifcation分类
轻工纺织引用本文复制引用
肖其胜,杨捷琳,杨惠琴,丁卓平,何宇平..实时荧光PCR法鉴定食品中双歧杆菌[J].食品科学,2016,37(20):177-182,6.基金项目
上海市科委长三角联合科技攻关项目（14495810200）；国家质检总局项目 （）
