肉类研究2016，Vol.30Issue(10)：30-34,5.DOI:10.15922/j.cnki.rlyj.2016.10.006
液相色谱串联质谱法快速测定猪肉中克伦特罗残留量
Fast Determination of Residual Clenbuterol in Pork by Liquid Chromatography with Tandem Mass Spectrometry
摘要
Abstract
A method was developed for determining residual clenbuterol in pork by liquid chromatography with tandem mass spectrometry. Five grams of pork samples were extracted with ethyl acetate under basic condition. Then, clenbuterol was extracted from the extract using formic acid solution. After defatting with hexane, the extract was directly used for determination of clenbuter by liquid chromatography with tandem mass spectrometry (LC-MS-MS) on an Acquity BEH C18 column with a mixture of 0.1% formic acid solution and methanol as the mobile phase under gradient elution conditions. The mass spectrometer was operated in multiple reaction monitoring (MRM) mode using positive electrospray ionization. The analyte was quantiifed with the isotope dilution and internal standard methods. Good linearity was obtained for clenbuterol in the concentration range of 0.05–10.0μg/L with correlation coefifcient more than 0.999. The recoveries of pork samples were 95.9%–101.5%at fortiifed levels of 0.25–0.75μg/kg. The proposed method exhibited a limit of detection of 0.10μg/kg and a limit of quantitation of 0.25μg/kg for clenbuterol. The relative standard deviations of intra-assay and inter-assay precision were between 3.3% and 4.6%, and between 4.1% and 5.1%, respectively. The method is demonstrated to be suitable for the fast determination of clenbuterol in pork.关键词
克伦特罗/快速测定/液相色谱串联质谱法/残留量Key words
clenbuterol/fast determination/liquid chromatography with tandem mass spectrometry (LC-MS-MS)/residue分类
轻工纺织引用本文复制引用
张艳,陈国,吕燕,吴银良..液相色谱串联质谱法快速测定猪肉中克伦特罗残留量[J].肉类研究,2016,30(10):30-34,5.基金项目
宁波市重大科技攻关项目 （）
