茶叶科学2016，Vol.36Issue(6)：567-574,8.
茶园芽孢杆菌QM7促生特性及耐酸铝机制初步研究
Research on The Promoting Effects on Tea Growth and Aluminum Tolerant Mechanism of Bacillus subtilis Strain QM7
摘要
Abstract
At present, the biological activities of Bacillus.spp and other microorganisms in the acid tea garden soil are not high, thereby difficult to effectively promote plant growth and replace or reduce the usage of chemical fertilizer. In this study, one of Aluminum (Al) tolerance strains, Bacillus subtilis QM7 was isolated from tea garden soil. The auxin (IAA) production of QM7 was influenced by Al concentration (from 85.6 mg·L-1 to 36.8 mg·L-1 under 0 to 1 mmol·L-1 external Al respectively). Results of pot experiment showed that strain QM7 could promote plant growth by increasing root surface area by 56.8%and numbers of root tips by 27.3%in 6 weeks. Two-dimensional gel electrophoresis (2-DE) was used to identify proteins involved in Al toxicity-induced changes. Results showed that more than 64 proteins differentially expressed in response to Al stres s, including proteins involved in DNA transcription, protein translation and cell envelope, which reduced the growth of Bacillus subtilis.关键词
茶园土壤/耐酸铝芽孢杆菌/生长素(IAA)/蛋白电泳Key words
tea planting soil/aluminum tolerance Bacillus subtilis/auxin (IAA)/two-dimensional gel electrophoresis分类
农业科技引用本文复制引用
罗毅,苏有健,张永利,夏先江,宋莉,王烨军,廖万有..茶园芽孢杆菌QM7促生特性及耐酸铝机制初步研究[J].茶叶科学,2016,36(6):567-574,8.基金项目
国家茶叶产业技术体系土壤肥料岗位项目（CARS-23-07B）、安徽省农业科学院科技创新基金项目（16A0823）、安徽省现代农业茶产业技术体系茶树栽培岗位项目（2016N1820）。 （）
