茶叶科学2016，Vol.36Issue(6)：655-662,8.
茶黄素与EGCG抑制体内外β-淀粉样蛋白1-42水平及其诱导的神经细胞氧化损伤
Theaflavins and EGCG Protect SH-SY5Y Cells from Oxidative Damage Induced by Amyloid-β1-42 and Inhibit the Level of Aβ42 in vivo and in vitro
摘要
Abstract
In this study, amyloid β-protein Aβ42 was incubated with EGCG, TF, TF-3-G, TF-3'-G, TF-DG at the ratio of 1:1 and 1:5 under simulated physiological condition. The contents ofβ-sheets after incubation were tested by thioflavin T fluorometric assay. It was found that EGCG and theaflavins could suppress the formation ofβ-sheet structure, thereby inhibiting aggregations of Aβ42. In addition, an SH-SY5Y cell damage model was established and MTT assay was used to examine cell viabilities when 20 μmol·L-1 Aβ42 was incubated with different concentrations of EGCG and theaflavins (10, 50, 100μmol·L-1). Intracellular reactive oxygen species (ROS) levels, antioxidant enzyme activities (GSH-Px) and MDA levels were also measured. The results showed EGCG and theaflavins treatments could alleviate the Aβ42 induced oxidative damage to neural cells by reducing ROS and increasing the activity of GSH-Px. To study the in vivo protective effects of EGCG and theaflavins, SAMP8 mice were treated with 10 mg·kg-1·d-1 EGCG or theaflavins respectively by gavage for 10 weeks, and the contents of Aβ42 and AGEs in mouse serum were determined. The results showed that EGCG and theaflavins significantly decreased the content s of Aβ42 and AGEs in SAMP8 serum. In summary, EGCG and theaflavins could inhibit the aggregations of Aβ42 and reduce oxidative injury induced by Aβ42 in neural cells, thereby exerting protective effects on AD.关键词
EGCG/茶黄素/β-淀粉样蛋白1-42/晚期糖基化终末端产物/SH-SY5Y细胞/SAMP8小鼠Key words
EGCG/theaflavins/amyloid-β1-42/advanced glycation end products/SH-SY5Y cells/SAMP8 mice分类
轻工纺织引用本文复制引用
张静,黄建安,蔡淑娴,易晓芹,刘建军,王英姿,田丽丽,刘仲华..茶黄素与EGCG抑制体内外β-淀粉样蛋白1-42水平及其诱导的神经细胞氧化损伤[J].茶叶科学,2016,36(6):655-662,8.基金项目
国家茶叶产业技术体系项目（CARS-23-11B）、湖南省植物功能成分利用协同创新项目（HNCR-2014003）、国家自然科学基金项目（31471590）、湖南省研究生科研创新项目（CX2015B245）。 （）
