4株猪流行性腹泻病毒S1基因生物信息学分析OA北大核心CSTPCD
Bioin for matics Analysis on S1 Gene of Four Porcine Epidemic Diarrhea VirusStrains
为分析猪流行性腹泻病毒(porcine epidemic diarrhea virus,PEDV)的遗传变异情况,本研究设计合成1对引物,应用RT-PCR方法扩增S1基因的主要中和表位区序列,并对4株PEDV分离株S1基因中和表位区进行遗传进化和B细胞抗原表位分析.结果显示,4株分离株位于同一进化分支G2上,其中湖南株和河南株与JXGZ2013、GDZQ2012、GDZQ2014株亲缘关系最近,同源性高达98.3%~98.7%;上海株与BJ-20…查看全部>>
To analyse the genetic variation of porcine epidemic diarrhea virus (PEDV),one pair of primers was designed and RT-PCR was used to amplify the S1 gene epitope sequences of 4 PEDV field strains.Phylogenetic analysis showed that 4 strains were closely related to each other and belonged to the second group,and the Hunan and Henan isolates had a close relationship with JXGZ2013,GDZQ2012,GDZQ2014 strains,with the nucleotide homologies at 98.3% to 98.7%;Shanghai s…查看全部>>
朱海侠
福州大北农生物技术有限公司,福州 350014
农业科技
猪流行性腹泻病毒遗传进化B细胞抗原表位
porcine epidemic diarrhea virusgenetic variationB-cell epitope
《中国畜牧兽医》 2016 (11)
3024-3030,7
福州大北农生物技术有限公司畜禽常见病免疫防控技术研究专项基金
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