食品与机械2016，Vol.32Issue(11)：13-16,4.DOI:10.13652/j.issn.1003-5788.2016.11.003
微孔板法筛选α-葡萄糖苷酶抑制剂的条件优化
Optimization of a microplate bioassay method of filtering alpha-glucosidase inhibitors
王红月 1陈中 1简旭凤2
作者信息
- 1. 华南理工大学食品工程与科学学院，广东 广州 510640
- 2. 安利日用品公司，广东 广州 510730
- 折叠
摘要
Abstract
The half inhibitory concentration (IC 50 )of alpha glucosi-dase inhibitors was detected based on a microplate assay method in this study in order to find the effective inhibitors for preparing hypo-glycemic functional food.The reaction conditions were optimized by the four aspects,i.e.,the enzyme concentration,the detection wave-length, the substrate concentration and the reaction time.The results showed that the optimum enzyme concentration ranged from 2.0 mg/mL to 3.0 mg/mL,and waves 520 nm and 492 nm were opti-mum among the five waves, 405, 450, 492, 520, 630 nm. Moreover,the suitable substrate concentration of sucrose ranged from 20 mmol/L to 40 mmol/L,and the appropriate reaction time was about 20~30 min.关键词
α-葡萄糖苷酶抑制剂/血糖/微孔板法Key words
alpha glucosidase inhibitors/blood glucose/microplate bi-oassay引用本文复制引用
王红月,陈中,简旭凤..微孔板法筛选α-葡萄糖苷酶抑制剂的条件优化[J].食品与机械,2016,32(11):13-16,4.
