食品与机械2016，Vol.32Issue(11)：21-24,89,5.DOI:10.13652/j.issn.1003-5788.2016.11.005
81株铜绿假单胞菌16SrRNA基因序列测定及系统发育学分析
Identification of 81 Pseudomonas aeruginosa by phylogenetic analysis of 16S rRNA gene sequence
曾晓琮 1周露 2苏妙贞 1韩志杰 2陈丹霞1
作者信息
- 1. 广东省食品检验所，广东 广州 510435
- 2. 广东省酒类检测中心，广东 广州 510435
- 折叠
摘要
Abstract
81 P seudomonas aeruginosa from Guangdong Provincial Institute of Food Inspection were identified by 1 6S rRNA sequence a-nalysis.The DNA was isolated and the sequences of 1 6S rRNA gene were amplified by PCR with the bacterium universal primers,and then the PCR products were sequenced after 2% agarose gel electro-phoresis.Moreover, the corrected sequences were aligned with Clustal X and the phylogenetic tree was constructed by MEGA5.1 . Consequently,the identified results of the 81 strains confirmed their original identification before.On the phylogenetic tree,No.24-3-QY strain formed a separate branch with No.100-5-JM strain and No. 106-3-JM strain.No.2 8-1-DW formed one branch and the other 77 strains formed a separate branch with P .aeruginosa ATCC 27853. This was the first research about waterborne P .aeruginosa in Guangdong Province when China began to implement GB 1 9298—2014 “National food safety standards of packaged drinking water”since May 24, 2015.The waterborne P .aeruginosa culture collection in Guangdong was preliminarily established basing on these strains.These data will provide a powerful tool for effectively tracing source of P . aeruginosa and controlling the water contamination in future.关键词
铜绿假单胞菌/16SrRNA基因/序列分析/系统发育树Key words
P seudomonas aeruginosa/16SrRNA gene/gene sequence analysis/phylogenetic tree引用本文复制引用
曾晓琮,周露,苏妙贞,韩志杰,陈丹霞..81株铜绿假单胞菌16SrRNA基因序列测定及系统发育学分析[J].食品与机械,2016,32(11):21-24,89,5.
