食品与机械2016，Vol.32Issue(11)：25-28,34,5.DOI:10.13652/j.issn.1003-5788.2016.11.006
天麻提取物对灰树花深层发酵胞外蛋白合成的影响
Effect of Gastrodia elata Bl .extracts on the biosynthesis of extracel lular proteins in the Grifola frondosa under submerged fermentation
摘要
Abstract
Gastrodia elata Bl.extracts was added into the system of Grifola frondosa submerged fermentation,and then supplement-ation of Gastrodia elata Bl .extracts were optimized according to the biomass and the synthetic quality of extracellular proteins in G . frondosa .The changes of components in G .elata Bl.extracts before and after the sterilization,including gastrodin,p-hydroxybenzyl alco-hol, p-hydroxybenzaldehyde, and parishin, were analyzed using HPLC.Moreover,the relationship of this changes between G .elata Bl.extracts and the in G .frondosa fermentation system were also investigated.The results revealed that 7% (v/v )G .elata Bl. extracts could facilitate G . frondosa to grow and produce extracellular proteins,and the content of two experimental indexes reached the maximum 1 1 days after the submerged fermentation of G .frondosa .It was found that the content of parishin in the extracts from G .elata Bl.decreased after sterilization,while the content of gastrodin increased obviously.Furthermore,the extracts from G . elata Bl.were added into the system of G .frondosa submerged fer-mentation and then they were incubated for 14 days.Finally,the contents of p-hydroxybenzyl alcohol and p-hydroxybenzaldehyde de-creased,nevertheless parishin increased by 1 6 1 .1 1 %.关键词
天麻/提取物/灰树花/深层发酵/胞外蛋白/生物量Key words
Gastrodia elata Bl . extracts/Grifola frondosa/submerged fermentation/extracellular protein/biomass引用本文复制引用
黄忠,吴天祥,杨祖滔,朱思洁..天麻提取物对灰树花深层发酵胞外蛋白合成的影响[J].食品与机械,2016,32(11):25-28,34,5.基金项目
国家自然科学基金资助项目(编号:[2014]31460537) （编号:[2014]31460537）
