食品与机械2016，Vol.32Issue(11)：174-177,4.DOI:10.13652/j.issn.1003-5788.2016.11.040
植物乳杆菌Lp-S2直投式发酵剂及其离心、冷冻干燥工艺研究
Study on centrifugal and freeze drying of Lactobaci l lus plantarum Lp-S2 DVS
摘要
Abstract
Lactobacillus plantarum Lp-S2 which has the ability to produce gamma-aminobutyric acid,was used as starting strain to de-velop Direct vat set (DVS).Then the centrifugation process and freeze-drying process were optimized.By orthogonal experiment the optimal conditions of centrifugation were identified as:centrifugal temperature 4 ℃,Time-consuming 30 minutes,centrifugal force 6 000 r/min.In this condition,the centrifugal recovery rate of Lac-tobacillus Lp-S2 is 83.8%.The cryoprotectant was optimized by single factor and orthogonal test.The best combination of cryopro-tectants was 1 5% trehalose,1 5% glucose and 10% skim milk pow-der.The survival rate of L .plantarum Lp-S2 Direct Vat Set reached 39.2%.The optimum pre-cooling time was 6 h.The number of viable cells of the direct-acting starter was 1 .5×10 1 2 CFU/mL.关键词
直投式菌剂/植物乳杆菌/冷冻干燥/γ-氨基丁酸Key words
directed vat set/Lactobacillus plantarum/freeze drying/γ-amino butyric acid引用本文复制引用
隋春光,梁金钟,王风青..植物乳杆菌Lp-S2直投式发酵剂及其离心、冷冻干燥工艺研究[J].食品与机械,2016,32(11):174-177,4.基金项目
黑龙江省高校科技创新团队建设计划项目(编号:2010td04) （编号:2010td04）
