食品与机械2016，Vol.32Issue(12)：35-39,5.DOI:10.13652/j.issn.1003-5788.2016.12.008
α-半乳糖苷酶辅助饱和硫酸铵法制备IgG及其活性研究
The preparation of IgG combining the method of Alpha galactose glucoside enzyme with saturated ammonium sulfate and its activity research
摘要
Abstract
The optimized preparation conditions of the concentration of IgG in yak blood after salting out of saturated ammonium sulfate by single factor experiments,including the selection of the hydrolysis time,pH,temperature and the amount of enzyme,as well as the Central Composite experiment,was investigated in this study.The activity of IgG was detected by ELISA and analyzed by using the Re-sponse Surface analysis (RSA).The results showed that the optimal concentration of IgG was 2 7 .1 3 mg/mL when hydrolyzed for at 3 9 ℃for 2 h,and enzymolyzed at pH 4.4,using an enzyme dosage 5 mL (467.5 U),and the content of IgG was 22.35 mg/mL improve 21.28% (P<0.05)under this condition.Moreover,it was also found that the activated content of IgG was changed from 16.07 mg/mL to 15.92 mg/mL,decreased by 0.93%,after the treatment of enzyme.关键词
牦牛血/α-半乳糖苷酶/饱和硫酸铵/免疫球蛋白 G (IgG)/活性Key words
yak blood/a-galactosidase/saturated ammonium sulfate/immunoglobulin G/activity引用本文复制引用
肖雪莉,张珍,张盛贵,徐荣荣,狄蕊吕,锦第,刘雅..α-半乳糖苷酶辅助饱和硫酸铵法制备IgG及其活性研究[J].食品与机械,2016,32(12):35-39,5.基金项目
甘肃省财政厅高校基本业务项目(编号:1011JKCA179) （编号:1011JKCA179）
甘肃省农牧厅生物技术专项(编号:GNSW-2013-22) （编号:GNSW-2013-22）
甘肃农业大学导师基金项目 （）
甘肃省自然基金(编号:1107RGZA23) （编号:1107RGZA23）
